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Migrates at approximately the same rate as 300-500bp DNA… Bromophenol blue, Tracking dye (CAS 115-39-9) (ab146339) | Abcam Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. https://www.scbt.com/scbt/product/bromophenol-blue-115-39-9, https://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=8272&loc=ec_rcs, http://www.sigmaaldrich.com/Graphics/COfAInfo/SigmaSAPQM/SPEC/B0/B0126/B0126-BULK________SIAL_____.pdf, "Agarose gel electrophoresis (basic method)", "Quantification of dichromatism: a characteristic of color in transparent materials", https://en.wikipedia.org/w/index.php?title=Bromophenol_blue&oldid=984535214, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 October 2020, at 16:16. All rights reserved. [7] Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index. 2019 Mar 22; 540: 148-154.

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Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB,[2] albutest[3]) is used as a pH indicator, an electrophoretic color marker, and a dye. PubChem Compound. © 1998-2020 Abcam plc. Bromphenol Blue - Use and Manufacturing. Store under desiccating conditions. Please fill out this form to request the QC report. At neutral pH, the dye absorbs red light most strongly and transmits blue light. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. We do not sell to patients.

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It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible.

ab146339 has not yet been referenced specifically in any publications. We have received your request and will respond to you as soon as possible. If you have published this work, please enter the PubMed ID. The product can be stored for up to 12 months. Tracking dye for nucleic acid or protein electrophoresis. 2018 Oct 5; 203: 333-341. Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups.

We're doing our best to keep everyone healthy and safe in the workplace while also avoiding the interruptions to our day-to-day operations. Please visit our frequently asked questions (FAQ) page for more details. O=S1(OC(C2=CC(Br)=C(O)C(Br)=C2)(C3=CC(Br)=C(O)C(Br)=C3)C4=CC=CC=C41)=O. Preferential solvation bromophenol blue in water-alcohol binary mixture.

Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

It has also been used as an industrial d

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Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.

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Thank you for being a loyal MedChemExpress customer, we are here to assist you as needed. Xylene cyanol and orange G may also be used for this purpose.[6]. PubChem Compound. Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients. Solutions of the dye, therefore, are blue. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, bromophenol-blue-tracking-dye-ab146339.pdf. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. pH indicator (pH 3.0 (yellow) - 4.6 (blue)). Nanocellulose for gel electrophoresis.

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It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Dangui AZ, et, al.

Bromphenol Blue - Compound Summary. Bromophenol blue (115-39-9) Chemical Names and Synonyms. Please store the product under the recommended conditions in the Certificate of Analysis. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Reaxys. J Colloid Interface Sci. If you need to change the delivery plan for items ordered, please contact us via email [email protected].

* Required Fields. As the situation with COVID-19 continues to unfold in every community, MedChemExpress is responding to the uncertainty caused by this outbreak thoughtfully and cautiously. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Based on 1 publication(s) in Google Scholar. There are currently no Customer reviews or Questions for ab146339.Please use the links above to contact us or submit feedback about this product. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. Spectrochim Acta A Mol Biomol Spectrosc.

Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. This phenomenon is called dichromatic color. You will hear from us soon. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.[4].

Please contact us to place your order, or try again later. As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. Store at Room Temperature. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

Nanocellulose for gel electrophoresis. Preferential solvation bromophenol blue in water-alcohol binary mixture.

In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on …

Bromophenol blue is also used as a dye. Publishing research using ab146339? 2019 Mar 22; 540: 148-154. 2018 Oct 5; 203: 333-341. Generally, these will be useable for up to one month. DMSO : 50 mg/mL (74.63 mM; Need ultrasonic), Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline, Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution, Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline).

Santa Cruz Biotechnology, Inc. Bromophenol Blue (CAS 115-39-9) Properties.

Mendoza L, et, al. Solutions of the dye, therefore, are blue. [1]. Submission failed, please try again later. . Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6[1][2]. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Our Cookie Policy explains how you can opt-out of the cookies we use. For research use only. [2]. It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. To our knowledge, customised protocols are not required for this product. Migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. J Colloid Interface Sci. We use cookies to make our site as useful as possible. Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.